Murph
:)
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https://www.nature.com/articles/s41467-023-44432-3
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Creatine kinase results are too low in most people who have been confined to bed rest for extended periods.Muscle abnormalities worsen after post-exertional malaise
Is it really necessary to reinvent the wheel?Awesome line of research.
If these muscle cell changes are also present in me/cfs it might explain why some have found benefit from taking Branched Chain Amino Acids. Should have posted that a while ago. Forgot.It's the sort of visible, structural finding that people can understand. And it is logical in a very simple way even a lay person can grasp: ah, they can't move their muscles because something is wrong with the muscle.
Look at the necrosis they see here in panel B. Muscle cells are just dying in a spectacular fashion after exercise.:
I wonder if there is a SMN1 and SMN2 genes problem, called "Spinal Muscular Atrophy (SMA)"Could it be simply that the cells had very little ck to start with? I asked.
Any chance Creatine supplementation would make it to the muscles and help?I emailed Rob Wust to ask how you get high necrosis without lots of ck splashing around. Could it be simply that the cells had very little ck to start with? I asked.
To his credit, he replied. Here's his response.
Thanks for this. We were puzzled about this too, but we think that either we haven’t measured this at the right time (we would love to ask these very sick people back in the lab multiple times, but that’s logistically difficult), but another important aspect is that the creatine levels in skeletal muscle in these patients were already very low (see the results in our paper). Lastly, we don’t know how extensive the muscle damage really is, as our biopsies were only the size of a single grain of rice.
These are possible reasons of our findings.
Best wishes,
Rob
Here's the creatine thing he mentions. very low in muscle (blue) but high/normal in blood (red):
View attachment 53316
We should ask Rob Wüst.Any chance Creatine supplementation would make it to the muscles and help?
Hrd to know. it's already high in blood, so will making it even higher n blood lift levels in muscle? maybe?Any chance Creatine supplementation would make it to the muscles and help?
Rapamycin is a protein synthesis inhibitorMuscle cells do not synthesize creatine; they take up exogenous creatine by specific Na+-dependent plasma membrane transporters. We found that extracellular creatine regulates the level of expression of these creatine transporters in L6 rat muscle cells. L6 myoblasts maintained for 24 hr in medium containing 1 mM creatine exhibited 1/3rd of the creatine transport activity of cells maintained for 24 hr in medium without creatine. Down-regulation of creatine transport was partially reversed when creatine-fed L6 cells were incubated for 24 hr in medium lacking creatine. Down-regulation of creatine transport occurred independently of amino acid and glucose transport. Furthermore, the down-regulation of creatine transporters by extracellular creatine was slowed by inhibitors of protein synthesis. These results suggest that creatine induces the expression of a protein that functionally inactivates the creatine transporters. Regulation of creatine transport by extracellular creatine also was observed in L6 myotubes and in cultures of human myoblasts and myotubes. Hence, the activity of creatine transport represents another site for the regulation of creatine homeostasis.